Intshayelelo

Kwiilabhoratri zanamhlanje, ukuchaneka kwemisebenzi kubeka iimfuno ezikhulayo kwimpahla. Ngokukodwa xa kusetyenzwa ngezixa-mkhondo zolwelo, abaqhubi bahlala bejongene nemingeni emininzi. Ilebhu yesiNtu, ngelixa isaxabisekile kwimisebenzi yesiqhelo, inkulu kwaye ayichanekanga xa uphethe iithamo ezincinci zolwelo, zenza kube nzima ukuhlangabezana neemfuno ezingqongqo zokuchaneka kunye nococeko kwiimeko zovavanyo.

Uyilo oluchanekileyo oluthweswe izidanga lomthamo omncinci webhotile yedropha yenza ukuba ulwelo lulawuleke kwaye luthembeke.

Kutheni iLebhu ingenakwenza ngaphandle kweeBottles zeBurette eziNcinci?

Iibhotile ze-burette ezinomthamo omncinci zisetyenziswa kakhulu kwiilabhoratri kuba zibonisa iingenelo ezizodwa ngokubhekiselele kokuchaneka, ukhuseleko kunye nokusebenza kakuhle.

1. Ukuchaneka kwesakhono sokulinganisa

Ukuphinda-phinda kunye nokuchaneka kwemifuniselo kuxhomekeke kulwelo oluchanekileyo. Iibhotile ezikhethekileyo zedropper zinexabiso elincinci lempazamo kuluhlu kunezikhongozeli ezithweswe izidanga zemveli kwaye zinokuhlangabezana neemfuno zovavanyo kunye nezongezo zomkhondo ezilawulwa ngokuqinileyo.

2. Uyilo oluchasene nongcoliseko

Ibhotile ye-dropper yenzelwe i-screw-seal cap okanye i-piece-piece dropper tip, ephucula kakhulu ukutywinwa kunye nokuthintela ngokufanelekileyo iziqulatho ekuphupheni okanye kwi-oxidizing. Ngexesha elifanayo, xa kuthelekiswa nemisebenzi ye-pipette efuna ukutshintshwa kweengcebiso rhoqo, i-dropper tip ngokwayo igwema imisebenzi emininzi kwaye inciphisa amathuba okungcola okunqamlezayo, ukuphucula ukusebenza kakuhle kunye nokugcinwa kwezinto ezisetyenziswayo.

3. Ukhuseleko lwezinto eziphathekayo

Iibhotile zedropper esizithengisayo zenziwe ngeglasi ephezulu ye-borosilicate, ukumelana nobushushu kunye ne-corrosion-resistant, ilungele unyango oluphezulu lobushushu okanye i-asidi eyomeleleyo kunye ne-alkali reagents.

Iimeko zosetyenziso oluqhelekileyo

Iibhotile ze-burette ezinomthamo omncinci zisetyenziswa ngokubanzi kwiinkalo ezininzi zovavanyo ngokusebenza kwazo kunye nokuguquguquka, ngakumbi kwimisebenzi yovavanyo efuna ukuchaneka okuphezulu kokulawula ulwelo kunye nokusebenza ngokulula.

1. Uvavanyo lwebhayoloji yeemolekyuli

Kwimisebenzi yenqanaba le-molecular, ubunzima kunye nomthamo wee-reagents zichaphazela ngokuthe ngqo iziphumo zovavanyo. Iibhotile ze-Dropper zifanelekile kwi-DNA / i-RNA yokukhutshwa kunye nokugcinwa, kunye nobukhulu be-1ml buthintela ngokufanelekileyo ukuphuphuma kwesampuli kwaye kuququzelele ukugcinwa kwefriji. Kwi-enzyme okanye kwimisebenzi ye-antibody, iibhotile ze-3ml zingasetyenziselwa ukukhupha inani elichanekileyo le-reagents, ukuphepha ukulahlekelwa ngumsebenzi obangelwa ukukhenkceza ngokuphindaphindiweyo kunye nokunyibilika kweebhotile ezinkulu, kunye nokuqinisekisa ukuveliswa kunye nokuzinza kwezilingo.

2. Uhlalutyo lwekhemikhali

Ukulungiswa kwe-vial eqhelekileyo kuhlalutyo lobungakanani, ibhotile ye-5 ml yedropha ibonelela ngesithuba sokujongwa ngokulula kunye nokuqhathwa kwaye ifanelekile kumanqanaba amaninzi. Kwizinto ezithile ezinetyhefu okanye eziguquguqukayo, incam yokuthontsi yebhotile kunye noyilo lwentambo yokutywina luphucula ngokumangalisayo ukhuseleko lokuphatha kwaye lunciphisa umngcipheko wokuvezwa kwabasebenzi kunye nokunyuka kwerhasi.

3. Iilabhoratri zokufundisa

Kwiikholeji kunye nokufundiswa kwelebhu yesikolo samabanga aphakamileyo, ukuhanjiswa kwangaphambili kwe-reagent akukwazi nje ukunciphisa ngokufanelekileyo inkunkuma ye-reagent, kodwa nokunciphisa amathuba oqhagamshelwano ngqo lwabafundi kunye neekhemikhali eziyingozi kunye nokuphucula umgangatho wemfundo yokhuseleko. Iibhotile ezicacileyo ezinezikali zinceda abafundi ukuba baseke "ukuqonda umthamo" kunye nokwazisa "i-titration echanekileyo", kunye nokuphucula uqeqesho lwezakhono zokulinga.

Isikhokelo soKhetho

Phakathi kweempawu ezininzi kunye nezixhobo onokuthi ukhethe kuzo, ukuthengwa kwesayensi kunye nengqiqo yeebhotile zedropha ezinomthamo omncinci ophumelele isidanga kubalulekile ukuqinisekisa ukuthembeka kunye nokhuseleko lweziphumo zovavanyo.

1. Ingqiqo yokukhetha umthamo

Iimfuno zovavanyo opaque ziyalela ubungakanani beebhotile ezilungelelanisiweyo:

• 1ml/2mliibhotile zifanelekile kwii-reagents ezincinci zexabiso elinye, ukunciphisa inkunkuma kunye nokuququzelela ukugcinwa.
• 3mliibhotile zezona ziqhelekileyo kunye nobukhulu bendalo yonke, ezifanelekileyo kwiimvavanyo zemihla ngemihla kwi-liquid dispensing, umthamo ophakathi kunye nokulula ukuthwala.
• 5mliibhotile zifanelekile kwizisombululo eziqhelekileyo, ukuphepha ukuphinda uzalise kwakhona kunye nokuphucula ukusebenza kakuhle kovavanyo.

2. Ugxininiso lweparamitha ephambili

Inkqubo yokhetho kufuneka igxininise koku:

• Ukucaca komlinganiselo: Iibhotile ze-dropper ezikumgangatho ophezulu kufuneka zifakwe i-laser okanye ziprintwe ngokubambelela okuphezulu ukunqanda ukufiphala kwesikali kwiqondo eliphezulu lokucoca inzala okanye ukucocwa kunye nokuqinisekisa ukufundeka kwexesha elide.
• Ukutywinwa: Kunconywa ukuba uqhube uvavanyo olulula lwe-inversion ngaphambi kokuthenga kokuqala - gcwalisa ibhotile ngamanzi, ujike i-cap ngokuqinile kwaye uyiguqule kwiiyure ze-24 ukujonga ukuba kukho nayiphi na into yokuvuza, esetyenziselwa ukulinganisa imeko yangempela yokugcina.

3. Isilumkiso sokuphepha imigibe

Iimeko ezingqongileyo zaselabhoratri zibeka iimfuno eziphezulu kwizinto zesikhongozeli, kwaye le miba ilandelayo kufuneka igxininiswe:

• Iibhotile zeplastiki ezikumgangatho ophantsi zinokuqulatha izinto zeplastiki okanye i-organic solvent leachate, ngakumbi xa kugcinwa iasidi okanye ii-organic reagents, ezinokuthi zithande ukungcoliseka, ezichaphazela ubunyulu kunye nokhuseleko lweemvavanyo.
• Iimveliso zexabiso eliphantsi ezineempazamo zesikali esikhulu zinokukhokelela kwimithamo engachanekanga ye-spiking, enokuthi ibangele umfuniselo womfuniselo okanye ukungaphumeleli kokuphindaphinda, ngakumbi xa kuyilwa ukusabela okucingayo.

Ukuqukumbela

Iibhotile ezincinci zedropper ezithweswe izidanga azibonakali kodwa zidlala indima ebalulekileyo kwindawo ephucukileyo nesebenzayo yelabhoratri. Ngolawulo oluchanekileyo lwesikali / ukusebenza kokutywinwa okugqwesileyo kunye nezinto ezikhethwayo ezihambelana neekhemikhali, zibonelela ngesiqinisekiso esiphindwe kathathu "sokuchaneka + ukhuseleko + olusebenzayo" kwimisebenzi yovavanyo. Ezi gadget zisisiseko kodwa zibalulekile ziqinisekisa ukuthembeka kwedatha, imfezeko yeesampulu, kunye nokuphindaphinda kweenkqubo zovavanyo.

Abavavanyi kufuneka bakhethe umthamo kunye nemathiriyeli yeebhotile ngokufanelekileyo ngokwemisebenzi yovavanyo eyahlukeneyo ukuze kuphuculwe impumelelo yokusebenza kwaye baphephe iimpazamo ezingeyomfuneko okanye imingcipheko. I-vial ehambelana ngokuchanekileyo ingaba yinxalenye ephambili yempumelelo yovavanyo.