Intshayelelo

Iityhubhu zenkcubeko ye-screw ezilahlayo zidlala indima ebalulekileyo kwimisebenzi yaselabhoratri.Ukusetyenziswa kwabo ngokuchanekileyo akuthinteli kuphela ngokufanelekileyo ukungcoliseka kwesampulu, ukungcoliswa komnqamlezo kunye nokulahleka kwesampulu, kodwa kwakhona kuqinisekisa ukuchaneka kunye nokuthembeka kwedatha yovavanyo. Ke ngoko, kubaluleke kakhulu ukulawula inkqubo yokusebenza esemgangathweni ukuqinisekisa umgangatho weemvavanyo.

Esi sikhokelo sisebenza kwiinkqubo zokusebenza zeetyhubhu zenkcubeko ye-screw elahlayo esetyenziswa kwinkcubeko yeseli, iimvavanyo ze-microbiology, uvavanyo lwezonyango kunye nezinye iinkalo.

Ukulungiselela phambi kokusetyenziswa

Ukulungiswa okwaneleyo phambi kovavanyo linyathelo elibalulekileyo ekuqinisekiseni ukusebenza ngokugqibeleleyo kweetyhubhu zenkcubeko ye-screw elahlayo. Okokuqala, ukuthembeka kwepakethe yangaphandle kufuneka kuhlolwe ngononophelo nakuphi na ukuphuka okanye ukungaphumeleli kwetywina, okuyimfuneko ukugcina ubunyumba beetyhubhu. Iityhubhu ezipakishwe kakuhle kufuneka zigcinwe kwindawo eyomileyo, ecocekileyo ukuthintela ukufuma okanye ukungcoliseka.

Isimo sokuvala inzala akufunekanga singahoywa. Iityhubhu zenkcubeko ye-screw ezilahlwayo eziveliswa ngabavelisi abaqhelekileyo zidla ngokuvalwa yi-gamma irradiation okanye i-ethylene oxide, kwaye ipakethe kufuneka ibe nophawu olucacileyo lokuvala inzala kunye nomhla wokuphelelwa. Abasebenzi baselabhoratri kufuneka bajonge indlela yokuvala inzala kunye nomhla wokuphelelwa kwawo ukuqinisekisa ukuba iityhubhu zikwimeko efanelekileyo yokusetyenziswa.

Le miba ilandelayo kufuneka iqwalaselwe xa kukhethwa iinkcukacha:

1. Ukukhetha umthamo: Iityhubhu eziqhelekileyo ze-15ml zingakhethwa kwiimvavanyo zesiqhelo, ngelixa ubukhulu be-50ml bunconywa kwinkcubeko enkulu.
2. Iimpawu zezinto eziphathekayo: Izinto ze-polypropylene zinokumelana nobushushu obuphezulu, zifanelekile kwiimfuno zokuvala ubushushu obuphezulu; I-polystyrene imathiriyeli ibonakala ngokucacileyo, kulula ukuyiqwalasela.
3. Iimfuno ezizodwa: Kwiimvavanyo ezikhethekileyo, ezifana nokugcinwa kweqondo lokushisa eliphantsi, kufuneka ukhethe izinto ezinokumelana nobushushu obuphantsi.

Abasebenzi baselabhoratri kufuneka bakhethe eyona modeli ifanelekileyo yetyhubhu yenkcubeko ngokwemiqathango ethile yovavanyo, bethathela ingqalelo iimfuno zamandla e-centrifugal, ukuhambelana kweekhemikhali kunye nezinye izinto. Kucetyiswa ukuseka inkqubo yokusebenza esemgangathweni yokukhethwa kwezinto ezisetyenziswa kwibhubhoratri ukuze kuqinisekiswe ukuhambelana kunye nokuveliswa kovavanyo.

Inkqubo echanekileyo

1. Ukukhupha izinto

• Khulula iityhubhu zenkcubeko kwindawo yokusebenza ecocekileyo ukuqinisekisa ukuba akukho ngcoliseko yangaphandle ngexesha lokusetyenziswa.
• Nxiba iiglavu ezingenazintsholongwane okanye usebenzise i-tweezers ezinyumba ukususa iityhubhu xa ukhulula ukuphepha ukudibana ngqo neendawo ezibalulekileyo.

2. Ukusebenza ngespiking

• Kuvinjelwe ngokungqongqo ukuthintela udonga lwangaphakathi lwe-orifice okanye i-cap yangaphakathi yesivalo ngexesha lokuzaliswa kokuthintela ukuqaliswa kwemithombo yokungcola.
• Umthamo kufuneka ulawulwe xa ufaka iisampulu ezingamanzi kwaye akufanele ugqithise isikali esikhulu ukuthintela ukuchitheka okanye ukutywinwa kakubi kweesampulu ngexesha lokusebenza.

3. Indlela yokutywina

• I-screw cap kufuneka iqiniswe emva kokudibanisa isampuli ukuqinisekisa isitywina esipheleleyo. Ukutywinwa kunokuqinisekiswa ngokubangela ukuba kujongwe nakuphi na ukuvuza.
• Nika ingqwalaselo kumandla okujija aphakathi ukunqanda amandla agqithisileyo abangela ukuguga okanye ukuqhawuka komsonto, okunokuchaphazela ukusetyenziswa kwakhona okanye isiphumo sokutywinwa.

4. Ukumakisha nokurekhoda

• Sebenzisa i-solvent-resistant, waterproof, iilebhile zelebhu ezixhathisa amaxolo okanye iziphawuli ukuze uleyibhelishe ngokuchanekileyo ulwazi lwesampulu kwiindawo ezicocekileyo, ezomileyo zetyhubhu.
• Kuphephe ukusebenzisa iphepha leleyibhile eqhelekileyo okanye iipeni ze-inki ezinokuthi zichaphazeleke kukufuma ukubola ukuthintela ukulahleka kolwazi ngexesha logcino.

Izilumkiso ezisetyenziswayo

1. Ukuphepha ukosuleleka

• Imisebenzi yovavanyo kufuneka yenziwe kwindawo ecocekileyo, engenathuli, kwibhentshi ecocekileyo kakhulu okanye ikhabhinethi yokhuseleko lwebhayoloji iyacetyiswa.
• Ukunciphisa ixesha lokukhulula iityhubhu zenkcubeko, kwaye umsebenzi kufuneka ukhawuleze kwaye ulungelelaniswe ukunciphisa umngcipheko wokuba sesichengeni.
• Iityhubhu zenkcubeko ezizimeleyo kufuneka zisetyenziswe kuhlobo ngalunye lweesampulu, kwaye ukuxuba kunqatshelwe ngokungqongqo ukuthintela ukungcola kunye nokuphazamiseka kwiziphumo zovavanyo.

2. I-Centrifugation kunye nokugcinwa

• Phambi kwe-centrifugation, qiniseka ukuba uqinisekisa ukuba i-screw cap ikrwelwe ngokuqinileyo ukuthintela isampulu ukuba ingavuzi; wenze kwakhona umgangatho olungileyo we-symmetrical kwi-centrifuge ukuphepha ukungalingani koomatshini.
• Xa ugcina, iityhubhu kufuneka zibekwe kwindawo ethe tye ukuphepha ukuvuza okubangelwa kukubekwa okuthe tye. Gwema ukubeka iibhubhu kwiqondo lokushisa eliphezulu, ukukhanya okukhanyayo okanye indawo enomswakama ukuze ugweme ukuchaphazela ukuzinza kweesampuli kunye nokusebenza kweetyhubhu.

3. Ukuphathwa kwesampuli ekhethekileyo

• Iisampulu eziqulethe i-volatile, i-solvents eziphilayo okanye izinto ezinobungozi obunamandla, imodeli ekhethekileyo enokumelana neekhemikhali kufuneka isetyenziswe.
• Ukulungiselela i-cryopreservation, sebenzisa iityhubhu ze-cryopreservation ezichasene namaqondo okushisa aphantsi; Iityhubhu eziqhelekileyo ezilahlwayo zinokuba brittle okanye zivuze kumaqondo obushushu aphantsi kakhulu.

Yintoni omawuyenze emva kokusetyenziswa

1. Ukulungiswa kokhuseleko lwebhayoloji

• Iityhubhu zenkcubeko eziqulethe iisampulu zebhayoloji ezosulelayo, ezinokuba kho okanye ezinomngcipheko ophezulu webhayoloji kufuneka zifakwe ngokuzenzekela okanye zingasebenzi ngesibulali-ntsholongwane esisebenzayo ngokuhambelana neemfuno zenqanaba lebhubhoratri yokhuseleko phambi kokuba zilahlwe njengenkunkuma.
• Iityhubhu ezilahliweyo kufuneka zifakwe kumgqomo wenkunkuma “weplastiki engcoliswe ngebhayoloji” ngokohlelo kunye nenkqubo yolawulo lwenkunkuma eyingozi yelabhoratri, kwaye kufuneka ingaxutywa nenkunkuma eqhelekileyo ukuze ilahlwe.

2. Iingcebiso zokusingqongileyo

• Beka phambili iityhubhu zenkcubeko ezenziwe ngezinto ezinokuphinda zisetyenziswe kwaye uthathe inxaxheba kwisicwangciso esisembindini sokurisayikilishwa kunye nenkqubo yokulahlwa kokusingqongileyo kwizinto ezityiwayo kwiilabhoratri apho iimeko zivuma.
• Ukunciphisa inkunkuma engafanelekanga yezinto ezisetyenziswayo, khuthaza ukusetyenziswa okunengqiqo kwezinto ezilahlayo phantsi kwesiseko sokhuseleko, kwaye ukhuthaze ukwakhiwa kweelabhoratri eziluhlaza.

Imibuzo ebuzwa qho

1. Kufuneka ndenze ntoni ukuba andikwazi kuqinisa i-screw-in cap?

Okokuqala jonga ukuba imisonto ilungelelaniswe ngokufanelekileyo kwaye akukho zinto zangaphandle zibambekile kwimisonto. Musa ukunyanzela imisonto ukuba ikhulule njengoko oku kungonakalisa i-orifice okanye ikepusi. Ukuba isasilela ukutywina, ityhubhu yenkcubeko kufuneka itshintshwe enye entsha.

2. Ngaba iityhubhu zenkcubeko ezilahlwayo zingaphinda zisetyenziswe?

Ukusetyenziswa okuphindaphindiweyo akukhuthazwa. Ukutywinwa kunye nobunyumba beetyhubhu zenkcubeko ezilahlwayo akusayi kuqinisekiswa emva kokusetyenziswa, kwaye ukusetyenziswa kwakhona kunokubangela ungcoliseko, iziphumo ezithatha icala, okanye umonakalo wetyhubhu.

3. Ndingenza ntoni xa ndinovuza xa ndifaka i-centrifuging?

Khangela ukuba isiciko sijijelwe ngokuqinileyo kwaye sitywine kakuhle, kwaye uqinisekise ukuba iityhubhu zenkcubeko zilinganiswe ngokufanelekileyo phambi kokufakwa kwi-centrifugation. Kuphephe ukusebenzisa ngaphezu kwesantya esiphezulu se-centrifugal apho iityhubhu zilinganiswe khona. Ukuba kukho imfuneko, khetha ityhubhu ye-centrifuge ekhethekileyo echasene noxinzelelo njengenye indlela.

Ukuqukumbela

Ukusetyenziswa okusemgangathweni kweetyhubhu zenkcubeko ye-screw elahlayo linyathelo elibalulekileyo lokuqinisekisa ukuchaneka kweziphumo zovavanyo, ukhuseleko lwesampulu kunye nokuthembeka kokusebenza. Ukuthotyelwa okungqongqo kwimigangatho yokusebenza kunye neemfuno ze-biosafety, nokuba kukuqokelela isampuli, ukuphatha, ukugcinwa okanye ukulahla, isiqinisekiso esisisiseko sokuphucula umgangatho wokulinga kunye nokunciphisa ingozi.

Kucetyiswa ukuba amagcisa elebhu asebenzise iityhubhu zenkcubeko ezikumgangatho ophezulu ngokutywinwa kakuhle, ukumelana neekhemikhali kunye noluhlu lobushushu obusebenzayo ngokweemfuno zabo zovavanyo, ukuze kuphuculwe ukusebenza kakuhle kweemvavanyo kunye nokuqonda ulawulo oluzinzileyo lwezixhobo zelabhoratri.