Intshayelelo

Kwiilebhu zanamhlanje ze-biomedical kunye neekhemikhali,Iityhubhu zokukhulisa ezilahlwayo zisetyenziswa kakhulu kwinkcubeko yeeseli, kwiimpendulo zeekhemikhali, ekugcinweni kweesampuli nakwezinye iinkqubo ezibalulekileyo.Ukubaluleka kwazo kwinkqubo yovavanyo akunakutyeshelwa. Ekubeni ezi tyhubhu zokulima zidibana ngqo neesampuli zovavanyo, izinto zazo, ubungakanani, ukuvalwa kwazo nokuba zicociwe okanye azicocwanga kuya kuba nefuthe elikhulu kwiziphumo zovavanyo. Ukukhetha okungafanelekanga kunokukhokelela ekungcolisweni okunqamlezileyo, ukulahleka kwesampuli okanye ukukhetha idatha yovavanyo, ngaloo ndlela kuchaphazela ukuchaneka kunye nokuphindaphinda kophando.

Iintlobo eziphambili zeetyhubhu zenkcubeko ezilahlwayo

Kukho uluhlu olubanzi lweetyhubhu zokukhulisa izityalo ezilahlwayo, kwaye abaphandi kufuneka bakhethe uhlobo olufanelekileyo ngokwenjongo yovavanyo, indawo yokusebenza kunye neempawu zeesampuli. Zihlelwe ngokwamacandelo amathathu alandelayo: izinto, umthamo kunye nomsebenzi okhethekileyo:

1. Uhlu ngokwezinto eziphathekayo

Iityhubhu zenkcubeko ezilahlwayo ezenziwe ngezinto ezahlukeneyo zahlukile kukumelana nobushushu, uzinzo lweekhemikhali kunye neempawu zokukhanya:

• I-Polypropylene: Ukumelana okuhle nobushushu obuphezulu kunye nokugqwala kweekhemikhali, kufanelekile kwinkcubeko yesiqhelo yeeseli, uvavanyo lwebhayoloji yemolekyuli kunye nezinye izicelo.
• I-Polystyrene: ukukhanya okuphezulu, kulula ukuyibona imeko yolwelo kunye neseli, esetyenziswa rhoqo kuvavanyo lwe-optical, kodwa hayi ukumelana nobushushu obuphezulu, ngokuqhelekileyo ayinakusetyenziselwa ukuzivala ngokuzenzekelayo.
• Iityhubhu zenkcubeko yeglasiNangona zingasetyenziswa kwakhona kwaye zizinzile ngokweekhemikhali, zibiza kakhulu, zifuna iinkqubo ezongezelelweyo zokucocwa kunye nokubulala iintsholongwane, kwaye zibeka umngcipheko wokungcola okusasazekayo.

2. Uhlulo ngokwesixa somthamo

Ngokuxhomekeke kumthamo wesampulu ofunekayo kolu vavanyo, umthamo weetyhubhu zokukhulisa izityalo ususela kwi-micro ukuya kwi-volumes ezinkulu:

• Iityhubhu ze-Microcentrifuge: isetyenziswa kakhulu ekukhupheni iisampulu, ukuhla kwe-centrifugal, ukukhupha i-DNA/RNA kunye neminye imisebenzi.
• Iityhubhu zenkcubeko eziqhelekileyo: umthamo osetyenziswa kakhulu kwilebhu, ofanelekileyo kwinkcubeko yeseli, ukuxuba i-reaction, ukugcinwa kwesampuli kunye nezinye iinjongo.
• Iityhubhu zenkcubeko ezinomthamo omkhulu: ifanelekile kwinkcubeko yamaseli amakhulu okanye ekusetyenzweni kwesisombululo esikhulu.

3. Uhlulo ngokwemisebenzi ekhethekileyo

Iityhubhu ziyafumaneka zineempawu ezahlukeneyo ezongezelelweyo ukuhlangabezana neemfuno ezithile zovavanyo:

• Iityhubhu eziSetyhidiweyo eziSetyhidiweyo: i-fektri ehlanjululwe nge-gamma radiation okanye i-autoclaving, ifanelekile kwiimvavanyo ezineemfuno eziphezulu ze-aseptic.
• Ngesiciko sekhatriji: ivumela ukutshintshiselana ngegesi, ilungele iintsholongwane okanye iiseli ezifuna umoya kwaye ithintela ungcoliseko oluvela kwimithombo yangaphandle.
• Iityhubhu Ezimelana Nobushushu Obuphantsi: ingasetyenziswa ngokukhuselekileyo kwindawo ene-nitrogen engamanzi -80℃ okanye nakwindawo ene-nitrogen engamanzi, ifanelekile ukugcinwa kweesampuli zebhayoloji kubushushu obuphantsi bexesha elide.
• Iityhubhu ezigqityiweyo/ezingagqitywangaIityhubhu ezigqityiweyo zilungele ukuqikelela ngokukhawuleza kunye nokukhupha umthamo wolwelo ukuphucula ukusebenza kakuhle kwezilingo.

Izinto ezibalulekileyo ekukhetheni iityhubhu zenkcubeko

Kwinkqubo yoyilo kunye nokusebenza kovavanyo, ukukhethwa kweetyhubhu zokulima ezifanelekileyo ezilahlwayo kubalulekile ukuqinisekisa ukusebenza kakuhle kovavanyo kunye nokuchaneka kweziphumo. Abaphandi kufuneka baqwalasele ngokupheleleyo izinto ezininzi ezibalulekileyo:

1. Uhlobo lovavanyo

Iimfuneko zeetyhubhu zokukhulisa izityalo zahluka kakhulu kuvavanyo ngalunye kwaye kufuneka zikhethwe ngokwemixholo yokusebenza kunye nendawo yovavanyo:

• Kwinkcubeko yeseli: iimfuneko zokungabi nantsholongwane ziphezulu kakhulu, kwaye ukusetyenziswa kweetyhubhu ezifakwe iintsholongwane ezisandula ukususwa ezinee-cartridge caps eziphefumlayo ukuqinisekisa ukuba kutshintshiselwana ngegesi kuyacetyiswa.
• Uvavanyo lwe-PCR / i-molecular biology: kufuneka ingabi na-DNA enzymes, ii-RNA enzymes kunye neetyhubhu ezingena-pyrogen, zihlala zisebenzisa iityhubhu ze-polypropylene ezicocekileyo.
• Indawo yokugcina ubushushu obuphantsi: Iityhubhu ezinokumelana kakuhle nobushushu obuphantsi kufuneka zisetyenziswe ukuthintela ukucolwa kobushushu kumaqondo obushushu aphantsi.

2. Iimpawu zesampulu

Iimpawu ze-physicochemical zesampuli zichaphazela ngokuthe ngqo ukhetho lwezinto kunye nolwakhiwo olusebenzayo lweetyhubhu zenkcubeko:

• Iisampuli ezingamanzi okanye eziqinileyo: imisela ubungakanani obufunekayo kunye nohlobo lwe-tube orifice.
• Iisampuli ze-asidi okanye ze-alkaline: iisampuli ezirhabaxa kakhulu zifuna izinto ezinganyangekiyo ngamakhemikhali ukuze kuthintelwe ukuguquka okanye ukungcola kweetyhubhu.
• Nokuba kuphephe ukukhanya na: iisampuli ezinobuthathaka ekukhanyeni kufuneka zikhethe ityhubhu yokukhulisa izinto eziluhlaza okanye ezingabonakaliyo, ukuze kuthintelwe ukubola kwe-photo.

3. Iimfuneko zokubulala iintsholongwane

Imfuneko yokubulala iintsholongwane kunye nohlobo lokubulala iintsholongwane olusetyenzisiweyo zezona zinto zibalulekileyo ekufuneka ziqwalaselwe xa kukhethwa iityhubhu:

• Ukubulala iintsholongwane ngaphambi kokubulala iintsholongwane ngokuchasene nokubulala iintsholongwane ngokwakhoIimveliso ezisandula ukubulala iintsholongwane zasefektri zifanelekile kwiimvavanyo eziphezulu, oko konga ixesha; ukuba ilabhoratri inezixhobo zokubulala iintsholongwane ze-autoclave, ungakhetha ityhubhu ye-PP enokuvuthelwa ngokwayo.
• Ukuhambelana kokubulala iintsholongwaneUmzekelo, izinto ze-PS azifanelekanga ukufakelwa i-autoclaving kwaye zenzelwe ukusetyenziswa kube kanye kuphela.

4. Ukuhambelana

Iityhubhu kufuneka zilungelelaniswe kakuhle nezixhobo zelebhu ukuqinisekisa ukusebenza kakuhle kunye neziphumo ezithembekileyo:

• Ukuhambelana kweCentrifugal: Iityhubhu ezisetyenziselwa i-centrifugation kufuneka zomelele ngokwesakhiwo ukuze zimelane namandla e-centrifugal kwisantya esiphezulu sokujikeleza.
• Ukuhambelana kokuzenzekelayo: Kwiimvavanyo ezifuna ukusetyenziswa kweerobhothi zokufaka imibhobho, iinkqubo zokuhambisa ezizenzekelayo, njl.njl., kufuneka kusetyenziswe ubungakanani beetyhubhu ezisemgangathweni.

5. Iindleko kunye nokuzinza

Ulawulo olufanelekileyo lweendleko kunye nokusetyenziswa kwezixhobo nako kubalulekile ekufezekiseni iimfuno zovavanyo:

• Ingasetyenziswa kwakhona xa ilahlwa: iityhubhu ezilahlwayo kulula ukuzilawula kwaye ziphephe ungcoliseko, zifanelekile kwiimvavanyo ezisebenzisa amandla aphezulu; iityhubhu zeglasi eziphinda zisetyenziswe zifanelekile kwiimvavanyo ezisisiseko ezinebhajethi encinci.
• Isikali sokuthenga: ukuthengwa ngobuninzi kunokunciphisa ixabiso leyunithi, elifanelekileyo kwiiprojekthi ezinkulu zexesha elide; iinkcukacha ezincinci nezenzelwe wena ziguquguquka ngakumbi kodwa zibiza kakhulu.

Iingcebiso zeZimo zeSicelo esiQhelekileyo

Ngokweemfuno ezithile zeentlobo ezahlukeneyo zovavanyo, ezi tyhubhu zokukhulisa ezilahlwayo zilandelayo ziyacetyiswa kwiimeko ezininzi eziqhelekileyo zokusetyenziswa, ngenjongo yokuphucula ukusebenza kakuhle nokuthembeka kovavanyo:

1. Ukulima iiseli

• Uhlobo olucetyiswayo: iityhubhu zokukhulisa iipolypropylene ezingenazintsholongwane ezineekhaphu zekhatriji ezinokuphefumla
• Isizathu: Izinto zePolypropylene azinazo iikhemikhali kwaye zihambelana kakuhle ne-biocompatibility, zifanelekile kwi-cell culture fluid. Isiciko sekhatriji sinokutshintshana kwegesi okusebenzayo, sithintele ungcoliseko lweentsholongwane, kwaye sihlangabezane nemeko-bume yegesi efunekayo ukuze iiseli zikhule.

2. Ukuphunyezwa kweemolekyuli ze-PCR/qPCR

• Iintlobo ezicetyiswayo: iityhubhu ze-PCR ezikhethekileyo ezingenayo i-nuclease, ezingenayo i-pyrogen okanye iityhubhu ze-microcentrifuge
• Isizathu: Iityhubhu zepolypropylene ezicocekileyo kakhulu, emva konyango olungqongqo, zinokuphepha ngempumelelo ukonakala kwesampuli okanye ukungcola, ukuqinisekisa iziphumo zokukhulisa ezichanekileyo nezithembekileyo. Uyilo oluncinci luyacetyiswa ukuphucula ukusebenza kakuhle kokudluliselwa kobushushu.

3. Indawo yokugcina ubushushu obuphantsi

• Uhlobo Olucetyiswayo: Ityhubhu yePolypropylene Engangenisi Ubushushu Obuphantsi Enoyilo Lokuqhekeka Okungaqandisi kunye neSivalo Sokuvala Isikrufu
• Isizathu: Ezi tyhubhu zingasetyenziselwa ukugcina iisampuli ixesha elide kwi--80°C okanye nakwi-nitrogen engamanzi, kwaye isakhiwo esikhethekileyo sithintela ityhubhu ukuba ingaqhekeki kwaye ivuthe isampuli. Ifanelekile ukugcinwa ixesha elide kwemigca yeseli, iisampuli zegazi, iiproteni okanye ii-nucleic acid.

4. I-Centrifuge

• Iintlobo ezicetyiswayo: iityhubhu zepolypropylene ezinyamezelayo kakhulu, uyilo olujikelezileyo okanye oluyilwe ngekona, kunye neerotor ze-centrifuge
• Isizathu: Iityhubhu zePP zinokumelana okuhle kwe-centrifugal kwaye zinokumelana namandla aphezulu e-centrifugal ngaphandle kokuguquka okanye ukuqhekeka. Umzantsi we-conical unceda ukuqokelela iiseli okanye ukuhla phakathi kwaye wonyuse izinga lokubuyela kwimeko yesiqhelo.

Iimpazamo eziqhelekileyo kunye neendlela zokuziphepha

Enyanisweni, ukungaphumeleli kovavanyo ngenxa yokukhethwa okungafanelekanga kweetyhubhu zokukhulisa izityalo kwenzeka ngamanye amaxesha. Iimpazamo ezininzi eziqhelekileyo kunye neengcebiso ezifanayo zezisombululo zidweliswe ngezantsi ukuze abaphandi babhekisele kuzo:

1. Ukusetyenziswa gwenxa kwezinto ezingamelani nobushushu obuphezulu kwi-autoclaving